Summary

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Report Overview

This is a standalone HTML report that can be shared via email, flash drive, Google Drive, Dropbox, etc. This report is intended to provide metrics on the overall technical performance of the analyzed DuplexSeq libraries. The metrics table is sortable on any column value and exportable. The figures can be saved exactly as displayed to SVG files. There is also an option available to save the report as a non-interactive PDF. Each library should be assessed based on the metric values observed relative to the metric values expected. Any samples deviating significantly from the group should be scrutinized to evaluate if the deviation is biological in origin or a technical outlier requiring further investigation.

Gauges, Value Boxes

Libraries

12

Mean Passing Filter Raw Reads

97,129,322

Min Peak Tag Family Size On-Target

2

Max Peak Tag Family Size On-Target

97

Assay Performance Metrics

Assay Performance Metrics

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Metrics Definitions

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Contact Information

TwinStrand Biosciences, Inc.

twinstrandbio.com

support@twinstrandbio.com

3131 Elliott Ave, Suite 750 • Seattle, WA 98121 • USA

(206) 202-TWIN

© Copyright 2021. All rights reserved.

RP4_Im_High_D2.1

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Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.

RP4_Im_High_D4.1

Row

Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.

RP4_Im_Low_D2.1

Row

Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.

RP4_Im_Low_D4.1

Row

Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.

RP4_Im_Medium_D2.1

Row

Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.

RP4_Im_Medium_D4.1

Row

Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.

RP5_Im_High_D2.1

Row

Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.

RP5_Im_High_D4.1

Row

Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.

RP5_Im_Low_D2.1

Row

Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.

RP5_Im_Low_D4.1

Row

Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.

RP5_Im_Medium_D2.1

Row

Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.

RP5_Im_Medium_D4.1

Row

Family Size Plot

The proportion of raw reads that contribute to single strand consensus sequences (SSCS) of various sizes is visualized for both SSCS's that are orphaned and for SSCS's that are members of a complete duplex consensus. The peak of both distributions is explained in the plot subtitles. Unlike the assay performance summary metric "Peak Tag Family Size On-Target", these plots are derived from all reads from the library regardless of their mapping coordinates.